Is the "Room of Tears" for confirming that a new pope is male?
The short answer
No. It is where the new pope changes into papal vestments before receiving his fellow cardinals' pledges of obedience and appearing on the loggia. Its name comes from new popes often weeping at the weight of the burden. The "Pope Joan" story is a legend serious historians dismiss.
The Room of Tears is not for examining the pope's body. It is where the new pope changes into papal vestments before receiving the cardinals' pledges of obedience and being presented to Rome and the world from the loggia above St. Peter's Square. It is nicknamed the Room of Tears because new popes have often been overcome with emotion at the heavy burden placed on them.
The story of "Pope Joan" is a legend, roundly dismissed by serious historians as unworthy of belief.